Populus GT43 family members group into distinct sets required for primary and secondary wall xylan biosynthesis and include useful promoters for wood modification.
Identifieur interne : 001B89 ( Main/Exploration ); précédent : 001B88; suivant : 001B90Populus GT43 family members group into distinct sets required for primary and secondary wall xylan biosynthesis and include useful promoters for wood modification.
Auteurs : Christine Ratke [Suède] ; Prashant Mohan-Anupama Pawar ; Vimal K. Balasubramanian ; Marcel Naumann ; Mathilda Lönn S Duncranz ; Marta Derba-Maceluch ; András Gorzsás ; Satoshi Endo ; Ines Ezcurra ; Ewa J. MellerowiczSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2015.
Descripteurs français
- KwdFr :
- Activation de la transcription (génétique), Bois (génétique), Bois (métabolisme), Clonage moléculaire (MeSH), Famille multigénique (MeSH), Glucuronidase (métabolisme), Gènes de plante (MeSH), Paroi cellulaire (métabolisme), Populus (génétique), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes au cours du développement (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Vecteurs génétiques (métabolisme), Végétaux génétiquement modifiés (MeSH), Xylanes (biosynthèse).
- MESH :
- biosynthèse : Xylanes.
- génétique : Activation de la transcription, Bois, Populus.
- métabolisme : Bois, Glucuronidase, Paroi cellulaire, Vecteurs génétiques.
- Clonage moléculaire, Famille multigénique, Gènes de plante, Régions promotrices (génétique), Régulation de l'expression des gènes au cours du développement, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Cell Wall (metabolism), Cloning, Molecular (MeSH), Gene Expression Regulation, Developmental (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Genetic Vectors (metabolism), Glucuronidase (metabolism), Multigene Family (MeSH), Plants, Genetically Modified (MeSH), Populus (genetics), Promoter Regions, Genetic (MeSH), Transcriptional Activation (genetics), Wood (genetics), Wood (metabolism), Xylans (biosynthesis).
- MESH :
- chemical , biosynthesis : Xylans.
- chemical , metabolism : Glucuronidase.
- genetics : Populus, Transcriptional Activation, Wood.
- metabolism : Cell Wall, Genetic Vectors, Wood.
- Cloning, Molecular, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Genes, Plant, Multigene Family, Plants, Genetically Modified, Promoter Regions, Genetic.
Abstract
The plant GT43 protein family includes xylosyltransferases that are known to be required for xylan backbone biosynthesis, but have incompletely understood specificities. RT-qPCR and histochemical (GUS) analyses of expression patterns of GT43 members in hybrid aspen, reported here, revealed that three clades of the family have markedly differing specificity towards secondary wall-forming cells (wood and extraxylary fibres). Intriguingly, GT43A and B genes (corresponding to the Arabidopsis IRX9 clade) showed higher specificity for secondary-walled cells than GT43C and D genes (IRX14 clade), although both IRX9 and IRX14 are required for xylosyltransferase activity. The remaining genes, GT43E, F and G (IRX9-L clade), showed broad expression patterns. Transient transactivation analyses of GT43A and B reporters demonstrated that they are activated by PtxtMYB021 and PNAC085 (master secondary wall switches), mediated in PtxtMYB021 activation by an AC element. The high observed secondary cell wall specificity of GT43B expression prompted tests of the efficiency of its promoter (pGT43B), relative to the CaMV 35S (35S) promoter, for overexpressing a xylan acetyl esterase (CE5) or downregulating REDUCED WALL ACETYLATION (RWA) family genes and thus engineering wood acetylation. CE5 expression was weaker when driven by pGT43B, but it reduced wood acetyl content substantially more efficiently than the 35S promoter. RNAi silencing of the RWA family, which was ineffective using 35S, was achieved when using GT43B promoter. These results show the utility of the GT43B promoter for genetically engineering properties of wood and fibres.
DOI: 10.1111/pbi.12232
PubMed: 25100045
Affiliations:
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Balasubramanian</name></author><author><name sortKey="Naumann, Marcel" sort="Naumann, Marcel" uniqKey="Naumann M" first="Marcel" last="Naumann">Marcel Naumann</name></author><author><name sortKey="Duncranz, Mathilda Lonn S" sort="Duncranz, Mathilda Lonn S" uniqKey="Duncranz M" first="Mathilda Lönn S" last="Duncranz">Mathilda Lönn S Duncranz</name></author><author><name sortKey="Derba Maceluch, Marta" sort="Derba Maceluch, Marta" uniqKey="Derba Maceluch M" first="Marta" last="Derba-Maceluch">Marta Derba-Maceluch</name></author><author><name sortKey="Gorzsas, Andras" sort="Gorzsas, Andras" uniqKey="Gorzsas A" first="András" last="Gorzsás">András Gorzsás</name></author><author><name sortKey="Endo, Satoshi" sort="Endo, Satoshi" uniqKey="Endo S" first="Satoshi" last="Endo">Satoshi Endo</name></author><author><name sortKey="Ezcurra, Ines" sort="Ezcurra, Ines" uniqKey="Ezcurra I" first="Ines" last="Ezcurra">Ines Ezcurra</name></author><author><name sortKey="Mellerowicz, Ewa J" sort="Mellerowicz, Ewa J" uniqKey="Mellerowicz E" first="Ewa J" last="Mellerowicz">Ewa J. Mellerowicz</name></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Gene Expression Regulation, Developmental (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Genetic Vectors (metabolism)</term><term>Glucuronidase (metabolism)</term><term>Multigene Family (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Transcriptional Activation (genetics)</term><term>Wood (genetics)</term><term>Wood (metabolism)</term><term>Xylans (biosynthesis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation de la transcription (génétique)</term><term>Bois (génétique)</term><term>Bois (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Famille multigénique (MeSH)</term><term>Glucuronidase (métabolisme)</term><term>Gènes de plante (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Populus (génétique)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes au cours du développement (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Vecteurs génétiques (métabolisme)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>Xylanes (biosynthèse)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Xylans</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Xylanes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Transcriptional Activation</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Activation de la transcription</term><term>Bois</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Genetic Vectors</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bois</term><term>Glucuronidase</term><term>Paroi cellulaire</term><term>Vecteurs génétiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Gene Expression Regulation, Developmental</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Multigene Family</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Famille multigénique</term><term>Gènes de plante</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes au cours du développement</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The plant GT43 protein family includes xylosyltransferases that are known to be required for xylan backbone biosynthesis, but have incompletely understood specificities. RT-qPCR and histochemical (GUS) analyses of expression patterns of GT43 members in hybrid aspen, reported here, revealed that three clades of the family have markedly differing specificity towards secondary wall-forming cells (wood and extraxylary fibres). Intriguingly, GT43A and B genes (corresponding to the Arabidopsis IRX9 clade) showed higher specificity for secondary-walled cells than GT43C and D genes (IRX14 clade), although both IRX9 and IRX14 are required for xylosyltransferase activity. The remaining genes, GT43E, F and G (IRX9-L clade), showed broad expression patterns. Transient transactivation analyses of GT43A and B reporters demonstrated that they are activated by PtxtMYB021 and PNAC085 (master secondary wall switches), mediated in PtxtMYB021 activation by an AC element. The high observed secondary cell wall specificity of GT43B expression prompted tests of the efficiency of its promoter (pGT43B), relative to the CaMV 35S (35S) promoter, for overexpressing a xylan acetyl esterase (CE5) or downregulating REDUCED WALL ACETYLATION (RWA) family genes and thus engineering wood acetylation. CE5 expression was weaker when driven by pGT43B, but it reduced wood acetyl content substantially more efficiently than the 35S promoter. RNAi silencing of the RWA family, which was ineffective using 35S, was achieved when using GT43B promoter. These results show the utility of the GT43B promoter for genetically engineering properties of wood and fibres. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25100045</PMID><DateCompleted><Year>2015</Year><Month>09</Month><Day>01</Day></DateCompleted><DateRevised><Year>2014</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Populus GT43 family members group into distinct sets required for primary and secondary wall xylan biosynthesis and include useful promoters for wood modification.</ArticleTitle><Pagination><MedlinePgn>26-37</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pbi.12232</ELocationID><Abstract><AbstractText>The plant GT43 protein family includes xylosyltransferases that are known to be required for xylan backbone biosynthesis, but have incompletely understood specificities. RT-qPCR and histochemical (GUS) analyses of expression patterns of GT43 members in hybrid aspen, reported here, revealed that three clades of the family have markedly differing specificity towards secondary wall-forming cells (wood and extraxylary fibres). Intriguingly, GT43A and B genes (corresponding to the Arabidopsis IRX9 clade) showed higher specificity for secondary-walled cells than GT43C and D genes (IRX14 clade), although both IRX9 and IRX14 are required for xylosyltransferase activity. The remaining genes, GT43E, F and G (IRX9-L clade), showed broad expression patterns. Transient transactivation analyses of GT43A and B reporters demonstrated that they are activated by PtxtMYB021 and PNAC085 (master secondary wall switches), mediated in PtxtMYB021 activation by an AC element. The high observed secondary cell wall specificity of GT43B expression prompted tests of the efficiency of its promoter (pGT43B), relative to the CaMV 35S (35S) promoter, for overexpressing a xylan acetyl esterase (CE5) or downregulating REDUCED WALL ACETYLATION (RWA) family genes and thus engineering wood acetylation. CE5 expression was weaker when driven by pGT43B, but it reduced wood acetyl content substantially more efficiently than the 35S promoter. RNAi silencing of the RWA family, which was ineffective using 35S, was achieved when using GT43B promoter. These results show the utility of the GT43B promoter for genetically engineering properties of wood and fibres. </AbstractText><CopyrightInformation>© 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ratke</LastName><ForeName>Christine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU), Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pawar</LastName><ForeName>Prashant Mohan-Anupama</ForeName><Initials>PM</Initials></Author><Author ValidYN="Y"><LastName>Balasubramanian</LastName><ForeName>Vimal K</ForeName><Initials>VK</Initials></Author><Author ValidYN="Y"><LastName>Naumann</LastName><ForeName>Marcel</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Duncranz</LastName><ForeName>Mathilda Lönnäs</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>Derba-Maceluch</LastName><ForeName>Marta</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Gorzsás</LastName><ForeName>András</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Endo</LastName><ForeName>Satoshi</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ezcurra</LastName><ForeName>Ines</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Mellerowicz</LastName><ForeName>Ewa J</ForeName><Initials>EJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>08</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014990">Xylans</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018507" MajorTopicYN="N">Gene Expression Regulation, Developmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005810" MajorTopicYN="Y">Multigene Family</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015533" MajorTopicYN="N">Transcriptional Activation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014990" MajorTopicYN="N">Xylans</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">genetic engineering</Keyword><Keyword MajorTopicYN="N">poplar</Keyword><Keyword MajorTopicYN="N">secondary wall</Keyword><Keyword MajorTopicYN="N">transgenic trees</Keyword><Keyword MajorTopicYN="N">wood development</Keyword><Keyword MajorTopicYN="N">xylan biosynthesis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>04</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>06</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>06</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>9</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25100045</ArticleId><ArticleId IdType="doi">10.1111/pbi.12232</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Balasubramanian, Vimal K" sort="Balasubramanian, Vimal K" uniqKey="Balasubramanian V" first="Vimal K" last="Balasubramanian">Vimal K. Balasubramanian</name><name sortKey="Derba Maceluch, Marta" sort="Derba Maceluch, Marta" uniqKey="Derba Maceluch M" first="Marta" last="Derba-Maceluch">Marta Derba-Maceluch</name><name sortKey="Duncranz, Mathilda Lonn S" sort="Duncranz, Mathilda Lonn S" uniqKey="Duncranz M" first="Mathilda Lönn S" last="Duncranz">Mathilda Lönn S Duncranz</name><name sortKey="Endo, Satoshi" sort="Endo, Satoshi" uniqKey="Endo S" first="Satoshi" last="Endo">Satoshi Endo</name><name sortKey="Ezcurra, Ines" sort="Ezcurra, Ines" uniqKey="Ezcurra I" first="Ines" last="Ezcurra">Ines Ezcurra</name><name sortKey="Gorzsas, Andras" sort="Gorzsas, Andras" uniqKey="Gorzsas A" first="András" last="Gorzsás">András Gorzsás</name><name sortKey="Mellerowicz, Ewa J" sort="Mellerowicz, Ewa J" uniqKey="Mellerowicz E" first="Ewa J" last="Mellerowicz">Ewa J. Mellerowicz</name><name sortKey="Naumann, Marcel" sort="Naumann, Marcel" uniqKey="Naumann M" first="Marcel" last="Naumann">Marcel Naumann</name><name sortKey="Pawar, Prashant Mohan Anupama" sort="Pawar, Prashant Mohan Anupama" uniqKey="Pawar P" first="Prashant Mohan-Anupama" last="Pawar">Prashant Mohan-Anupama Pawar</name></noCountry><country name="Suède"><noRegion><name sortKey="Ratke, Christine" sort="Ratke, Christine" uniqKey="Ratke C" first="Christine" last="Ratke">Christine Ratke</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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